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  高质量脂蛋白产品
 
Yiyuan biotechnology 是国内规模最大分离纯化血浆脂蛋白的研究所,汇集中山大学具有十年以上脂蛋白研究经验专家,为广大科学家提供品质精良试剂:
人源脂蛋白相关产品
      人低密度脂蛋白(Human LDL)
      氧化型低密度脂蛋白(Human Ox-LDL, OxLDL)
      高氧化程度低密度脂蛋白(Human High Ox-LDL, High OxLDL)
      乙酰化低密度脂蛋白(Human Ac-LDL, AcLDL)
      DiI标记氧化型低密度脂蛋白(Human DiI-Ox-LDL)
      DiI标记乙酰化低密度脂蛋白(Human DiI-Ac-LDL)
      DiI标记低密度脂蛋白(Human DiI-LDL)
      高密度脂蛋白(Human HDL) DiI标记高密度脂蛋白(Human DiI-HDL)
      极低密度脂蛋白(Human VLDL) DiI标记极低密度脂蛋白(Human DiI-VLDL)
      无脂蛋白血清(Human Lipoprotein-free Serum or Lipoprotein Deficient Serum
      抗人SR-A抗体(Anti-SR-A),抗CD36抗体(Anti-CD36),抗人Ox-LDL抗体,抗人ApoE抗体
      油红O染色试剂盒(Oil Red O Staining kits)
 
Human Low Density Lipoprotein (Human LDL. Cat. No. YB-001)
 
LDL is a large protein (MW 3,500 kDa) with a diameter of 25.8 nm.It is composed of approximately 20-25% protein and 75-80% lipid. The lipid portion can be further described as 9% free cholesterol, 42% cholesteryl ester, 20-24% phospholipid, and 5% triglyceride.
  Yiyuan biotechnology LDL, is isolated from blood bank produced human plasma, via ultra-centrifugation (1.019-1.063g/cc). It is purified via ultracentrifugationto homogeneity determined by agarose gel electrophoresis.
Our LDL is membrane filtered and aseptically packaged. Store @ 4ºC.
Oxidized Human LDL OxLDL (Cat. No. YB-002)
 
Our Human LDL is oxidized using Cu2SO4 (oxidant) in PBS. Oxidation is terminated by adding excess EDTA-Na2. Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of OxLDL migrates 2.0fold further than the native LDL.
 
Yiyuanbiotech Native-LDL(n-LDL), Acetylated-LDL(Ac-LDL) and Oxidized-LDL(ox-LDL) were loaded on a agarose gel and electrophoresed for 60 minutes. The lipoproteins were stained with Sudan Black(A and B). Oil red O stainning was used to determine the formation of foam cell. RAW264.7 were incubated with with 80μg/mL ox-LDL for 24hrs (C),
High Oxidized LDL (High OxLDL)
 
Human LDL is oxidized extensively oxidized with Cu2SO4 (oxidant) in PBS at 37°C. It is used to induce cell apoptosis/death and cell injure. More detail about this product is available by sending a e-mail to: yiyuanbiotech@126.com
Acetylated Low Density Lipoprotein(Ac-LDL)
 
LDL is acetylated with acetic anhydride and dialyzed. It is ultrafiltered through a membrane and packaged aseptically under nitrogen.
DiI-Ox-LDL
 
oxidized Low Density Lipoprotein, labeled with 1,1’-dioctadecyl – 3,3,3’,3’-tetramethyl-indocarbocyanine perchlorate, which is highly fluorescent lipophilic dyes that diffuse into the hydrophobic portion of the LDL complex without affecting the LDL-specific binding of the apoprotein. DiI-Ox-LDL is used to label both vascular endothelial cells and macrophages. It can be used to identify and/or isolate these cells from mixed cell populations and investigate uptake and binding of modified LDL by different cell types. DiI has been excited at 488nm or 514nm and the emission is detected at 565 nm.

Representive confocal images of suptake of DiI-ox-LDL in macrop-
hages from ApoE knockout mice.
DiI-Ac-LDL
 
Acetylated Low Density Lipoprotein, labeled with 1,1’-dioctadecyl – 3,3,3’,3’-tetramethyl-indocarbocyanine perchlorate(see DiI-Ox-LDL ). modified low-density lipoprotein (LDL) including Ox-LDL, Ac-LDL can bind to cell membrance via class A scavenger receptor (SR-A) , CD36 and LOX-1 etc. When cells are labeled with DiI-Ac-LDL, the lipoprotein is degraded by lysosomal enzymes and the DiI (fluorescent probe) accumulates in the intracellular membranes. It can be used to quantify uptake of modified LDL. It also be used to identify isolated cells including vascular endothelial cells, macrophages, and endothelial progenitor cells(EPC).

Confocal images indicate
binding of DiI-Ac-LDL in
macrophages membrance
DiI-LDL
 
Low Density Lipoprotein labeled with 1,1’-dioctadecyl – 3,3,3’,3’-tetramethyl-indocarbocyanine perchlorate(see DiI-Ox-LDL ).
Human High Density Lipoprotein, HDL
 
Human High Density Lipoprotein, HDL, (Cat. No. YB-003) is isolated from blood bank produced human plasma. It is purified via ultracentrifugation (d=1.063 ~1.21 g/mL) to homogeneity determined by agarose gel electrophoresis.
VLDL
 
VLDL (d= <1.006 g/mL) is isolated from human plasma by ultracentrifugation. The purified VLDL is ultrafiltered through a membrane and packaged aseptically under nitrogen.
anti-SRA and anti-CD36
 
The macrophage class A scavenger receptor (SR-A) and CD36 mediate the uptake of modified low-density lipoprotein (LDL) including Ox-LDL, Ac-LDL, anti-SR-A and anti-CD36 is an affinity purified goat polyclonal antibody raised against a peptide mapping within an internal region of SR-A of human origin.
   
       Western blot analysis of SR-A
       expression in THP-1 whole cell
       lysate
油红O染色试剂盒 (Oil Red O Staining kits)
 
Atherosclerotic lesions were measured by lipid deposition stained Yiyuan Biotechnology oil red O kit represented here in red within thoraco-abdominal aortas from ApoE mice high lipid diet, less atherosclerotic lesions (upper aortas) and many (lower aortas) atherosclerotic lesions.
 
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