| Yiyuan biotechnology 是国内规模最大分离纯化血浆脂蛋白的研究所，汇集中山大学具有十年以上脂蛋白研究经验专家，为广大科学家提供品质精良试剂：
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 无脂蛋白血清 (Human Lipoprotein-free Serum or Lipoprotein Deficient Serum  货号：YB-005) |
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LDL is a large protein (MW 3,500 kDa) with a diameter of 25.8 nm.It is composed of approximately 20-25% protein and 75-80% lipid. The lipid portion can be further described as 9% free cholesterol, 42% cholesteryl ester, 20-24% phospholipid, and 5% triglyceride. |
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Yiyuan biotechnology LDL, is isolated from blood bank produced human plasma, via ultra-centrifugation (1.019-1.063g/cc). It is purified via ultracentrifugationto homogeneity determined by agarose gel electrophoresis.
Our LDL is membrane filtered and aseptically packaged. Store @ 4oC. |
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Our Human LDL is oxidized using Cu2SO4 (oxidant) in PBS. Oxidation is terminated by adding excess EDTA-Na2. Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of OxLDL migrates 2.0fold further than the native LDL. |
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Yiyuanbiotech Native-LDL(n-LDL), Acetylated-LDL(Ac-LDL) and Oxidized-LDL(ox-LDL) were loaded on a agarose gel and electrophoresed for 60 minutes. The lipoproteins were stained with Sudan Black(A and B). Oil red O stainning was used to determine the formation of foam cell. RAW264.7 were incubated with with 80μg/mL ox-LDL for 24hrs. |
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Human LDL is oxidized extensively oxidized with Cu2SO4 (oxidant) in PBS at 37°C. It is used to induce cell apoptosis/death and cell injure. More detail about this product is available by sending a e-mail to: yiyuanbiotech@126.com |
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LDL is acetylated with acetic anhydride and dialyzed. It is ultrafiltered through a membrane and packaged aseptically under nitrogen. |
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oxidized Low Density Lipoprotein, labeled with 1,1’-dioctadecyl – 3,3,3’,3’-tetramethyl-indocarbocyanine perchlorate, which is highly fluorescent lipophilic dyes that diffuse into the hydrophobic portion of the LDL complex without affecting the LDL-specific binding of the apoprotein. DiI-Ox-LDL is used to label both vascular endothelial cells and macrophages. It can be used to identify and/or isolate these cells from mixed cell populations and investigate uptake and binding of modified LDL by different cell types. DiI has been excited at 488nm or 514nm and the emission is detected at 565 nm. |
Representive confocal images of suptake of DiI-ox-LDL in macrop-
hages from ApoE knockout mice. |
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Acetylated Low Density Lipoprotein, labeled with 1,1’-dioctadecyl – 3,3,3’,3’-tetramethyl-indocarbocyanine perchlorate(see DiI-Ox-LDL ). Modified low-density lipoprotein (LDL) including Ox-LDL, Ac-LDL can bind to cell membrance via class A scavenger receptor (SR-A) , CD36 and LOX-1 etc. When cells are labeled with DiI-Ac-LDL, the lipoprotein is degraded by lysosomal enzymes and the DiI (fluorescent probe) accumulates in the intracellular membranes. It can be used to quantify uptake of modified LDL. It also be used to identify isolated cells including vascular endothelial cells, macrophages, and endothelial progenitor cells(EPC). |
Confocal images indicate
binding of DiI-Ac-LDL in
macrophages membrance |
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Low Density Lipoprotein labeled with 1,1’-dioctadecyl – 3,3,3’,3’-tetramethyl-indocarbocyanine perchlorate(see DiI-Ox-LDL ). |
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Human High Density Lipoprotein, HDL, (Cat. No. YB-003) is isolated from blood bank produced human plasma. It is purified via ultracentrifugation (d=1.063 ～1.21 g/mL) to homogeneity determined by agarose gel electrophoresis. |
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VLDL (d= <1.006 g/mL) is isolated from human plasma by ultracentrifugation. The purified VLDL is ultrafiltered through a membrane and packaged aseptically under nitrogen. |
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Purified YB VLDL is labeled with the fluorescent probe, DiI, and reisolated by ultracentrifugation (d <1.006 g/mL). The resultant product is exhaustively dialyzed against phosphate buffered saline, (pH 7.4), sterilized by membrane filtration and then aseptically packaged in a solution containing phosphate-buffered saline at pH 7.4 and 0.1 mM EDTA-Na2.
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The macrophage class A scavenger receptor (SR-A) and CD36 mediate the uptake of modified low-density lipoprotein (LDL) including Ox-LDL, Ac-LDL, anti-SR-A and anti-CD36 is an affinity purified goat polyclonal antibody raised against a peptide mapping within an internal region of SR-A of human origin. |
Western blot analysis of SR-A
expression in THP-1 whole cell
lysate |
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